Kroncke BM, Horanyi PS, and Columbus L. Structural origins of nitroxide side chain dynamics on membrane protein α-helical sites. Biochemistry 49:10045–10060 (2010)
Brett Kroncke
Brett receives 2011-2012 GSAS Dissertation Year Fellowship
Brett received one of the 20 Graduate School of Arts & Sciences Dissertation Year Fellowships awarded this year. The fellowship includes a stipend for his final year of study in the laboratory. The awardees were selected first by their department and then by a college committee. Students are selected based on their academic performance, research accomplishments, and contributions to the university and scientific community.
Graduate Student
BS: Chemistry, University of Wisconsin-Madison (2007)
Brett worked with Dr. John Wright on ultrafast pulse recognition hardware and software design and implementation.
Brett aims to use quantitative information obtained from EPR spectra to improve structure and dynamics determination for α-helical membrane proteins. His work investigating the structure and dynamics of spin labels on a model membrane protein, LeuT, has provided a quantitative understanding of the EPR lineshape of detergent/lipid exposed nitroxides. Currently he is working with a model protein, TM0026, to assess the degree to which this understanding can improve paramagnetic relaxation enhancement (PRE) derived nuclear magnetic resonance (NMR) α-helical membrane protein structure determination.
Linda, Dan, and Brett attended Frontiers of NMR in Biology Keystone Symposium
Dan presented a poster entitled “NMR Backbone Assignment of Opa I: A mediator of host-Neisseria interactions” and Brett presented a poster entitled “Investigations towards improving solution NMR paramagnetic relaxation enhancement distance restraints for MTSSL labeled α-helical membrane proteins”. Both were invited to orally present poster previews and Dan received a travel award. The meeting was located in Big Sky, Montana and the snow was fantastic.
Brett and Dan present posters at 39th Southeastern Magnetic Resonance Conference
Brett and Dan presented posters at the 39th Southeastern Magnetic Resonance Conference (SEMRC) which was held at the University of Florida campus on October 22-24, 2010. The focus of the conference is on the exchange of ideas related to NMR-, EPR-, and MRI-based research as well as on the development of these techniques and new applications. Dan presented a poster entitled “Preparation and NMR backbone assignment of OpaI: A mediator of host Neisseria interactions”. Brett presented “Motional model of nitroxide spin label side chain dynamics on an α-helical membrane protein” and received a travel grant.
Brett presents at 2010 EPR Conference
Brett presented an invited talk entitled “Structural Origins of Nitroxide Side Chain Dynamics on Membrane Protein α‐Helical Sites” at the 14th In Vivo EPR Spectroscopy and Imaging and the 11th International EPR Spin Trapping/Spin Labeling.
Brett shares 1st palce in 3rd year poster session
On April 1st, the third year chemistry graduate students presented their research at the annual poster event. Each year faculty judges award the top three poster presentations monetary awards. This year, Brett shared 1st place with Isabel Green and Joanna Webb received 2nd place. Brett and Isabel will give an oral presentation to the Department on April 27th, 2010.
Graduate and Undergraduate Students Present Posters at National Meetings
This last February and March,
Dan Fox presented his NMR studies of Opa proteins at the Frontiers in NMR Biology Keystone Symposium
Brett Kroncke (in photo) presented his EPR studies and Chris Reyes presented his Opa refolding and NMR studies at the 53rd Biophysical Society Meeting.
Thien Nguyen presented her mixed micelle refolding studies at the 2009 NIGMS Workshop: Enabling Technologies for Structural Biology.