Ryan Oliver

Oliver RC, Lipfert J, Fox DA, Lo RH, Kim JJ, Doniach S, Columbus L. Tuning micelle dimensions and properties with binary surfactant mixtures
Langmuir. 30(44):13353-61 (2014)

Ryan Lo and Ryan Oliver are now Dr. Lo and Dr. Oliver

On May 18, 2014, Ryan Oliver and Ryan Lo received their PhD at the Chemistry Graduation Ceremony.

They successfully defended their theses earlier in the Spring semester. Dr. Ryan Lo’s thesis entitled “Spectroscopic Studies of Membrane Protein Structure, Dynamics, and Function” focused on investigations of Opa proteins involved in the engulfment of Neisseria gonorrhoeae.
Dr. Ryan Oliver’s thesis entitled “Structural determination of membrane mimics using small-angle X-ray and neutron scattering” focused on determining the properties of detergents that are important in stabilizing membrane proteins for structural and functional studies.

Postdoctoral Research Associate in Biological Small-Angle Scattering of the Neutron Sciences Directorate at Oak Ridge National Laboratories

MS: Chemistry, University of N. Carolina at Charlotte (2010)
BS: Chemistry, University of N. Carolina at Chapel Hill (2005)
Ryan worked with Dr. Joanna Krueger at UNC-Charlotte studying structures of protein complexes in solution using small-angle x-ray and neutron scattering.

Ryan is studying the physical determinants of membrane protein structure and dynamics. Detergent micelles are typically employed to solubilize and stabilize membrane proteins in a protein-detergent complex (PDC). Properties such as the matching of the hydrophobic dimensions between detergent micelle and membrane protein are key factors in stabilizing the protein’s fold, but a molecular understanding of protein-detergent interactions that stabilize a membrane protein fold is unknown. Ryan aims to investigate the geometry, structure, and dynamics of PDCs using biophysical methods such as NMR, EPR, and SAXS.

Contact Ryan Oliver

Ono and Oliver attend the 246th ACS National Meeting

Ryan and Tomihiro attended the 246th American Chemical Society National Meeting in Indianapolis, Indiana on September 8-12, 2013. Tomihiro was accepted to the Physical Chemistry Symposium Workshop for Undergraduate Chemistry Majors and presented his poster entitled “Modulating detergent micelle fluidity for membrane protein structural studies”.

Oliver RC, Lipfert J, Fox DA, Lo RH, Doniach S, Columbus L. Dependence of Micelle Size and Shape on Detergent Alkyl Chain Length and Head Group PLoS ONE. 8:e62488 (2013).

Ashton and Ryan present at the 2013 Huskey Research Exhibition

Ashton Brock presented a poster entitled “Influence of detergents on the solubilization and function of lipoprotein signal peptidase A: Connecting detergent and membrane protein physical properties” and was awarded 1st prize in the Physical Sciences and Mathematics Division.

Ryan Oliver presented a poster entitled “Modulating the physical properties of micelles using detergent mixtures for membrane protein investigations”

Ryan, Ryan, and Linda attend the FASEB Conference: Molecular Biophysics of Membranes in Snowmass, CO

This years FASEB Conference: Molecular Biophysics of Membranes was in Snowmass, CO and The University of Virgina was well represented with the Kasson, Tamm, Wiener, and Columbus laboratories represented. Ryan Oliver and Ryan Lo presented posters entitled “Modulating the physical properties of micelles using binary detergent mixtures for future membrane protein investigations” and “Effects of detergent, ionic strength, and temperature on the quality of β-barrel membrane protein NMR spectra”, respectively. Ryan Lo’s poster earned him an NSF Travel Award. The conference program was intense, but we managed some time for hiking.
Maroon Bells
Ryan Oliver and Linda
Ryan Lo and Ryan Oliver
Ryan O says the water is pretty cold.

Dan and Ryan honored at the 2012 Robert J. Huskey Research Exhibition

The Huskey Research Exhibition is an annual event which provides a forum for all graduate students in the Graduate School of Arts and Sciences and the School of Architecture to publicize their research. In addition, the event rewards students presenting superior research. The 12th Annual Huskey Research Exhibition occurred on March 21, 2012. This year, Dan Fox and Ryan Oliver received second place in the Physical Science and Mathematics Division. Ryan’s poster was titled “Investigating the relationship between physical properties of detergents and membrane protein structure determination” and Dan’s poster was entitled “NMR solution structure of Opa60: a Neisserial membrane protein that mediates host phagocytosis”.

Ryan, Ryan, and Linda attend the MidAtlantic BioSAXS Workshop

This April, Ryan Oliver, Ryan Lo, and Linda attended the Rigaku and UNC Chapel Hill sponsored MidAtlantic BioSAXS Workshop. The day-long workshop focused on the latest developments in BioSAXS technologies and applications.