Golda Harris

Graduate school at the University of Pennsylvania

Golda Harris
Chemistry major with biochemistry focus
Distinguished Major Program

As an undergraduate researcher, Golda studied the catalytic mechanism of TM1385, a phosphoglucose isomerase (PGI) enzyme from Thermotoga maritima. She aims to distinguish between two possible mechanisms of the enzyme-catalyzed isomerization; enediol intermediate or direct hydride shift through 1H NMR and 2D 1H-13C NMR spectroscopy. Mutational studies will also help to determine the roles of conserved active site residues in the catalytic mechanism. A better understanding of the mechanism used by the PGI from T. maritima will contribute to knowledge of the relationship between a particular PGI catalytic mechanism, the structure of the active site, and the overall fold of the enzyme.