To address the challenges in membrane protein structural biology
To investigate the structural determinants of bacterial pathogen - host interactions mediated by membrane proteins.
Specifically, the structures of neisserial Opa proteins, which interact with host proteins and are directly involved in bacterial pathogenesis, are of interest.
However, in order to investigate the structure of these proteins, as well as the complexes with the host receptors, methods for accelerating structure determination are being developed. [Read more→]
Fox, DA and Columbus, L. Protein Science. 22: 1133–1140 (2013). Membrane proteins in detergent micelles are large and dynamic complexes that present challenges for solution NMR investigations such as spectral overlap and line broadening. In this study, multiple methods are introduced to facilitate resonance assignment of β-barrel membrane proteins using Opa60 from Neisseria gonorrhoeae as [...]