To address the challenges in membrane protein structural biology
To investigate the structural determinants of bacterial pathogen - host interactions mediated by membrane proteins.
Specifically, the structures of neisserial Opa proteins, which interact with host proteins and are directly involved in bacterial pathogenesis, are of interest.
However, in order to investigate the structure of these proteins, as well as the complexes with the host receptors, methods for accelerating structure determination are being developed. [Read more→]
Ryan H. Lo, Brett M. Kroncke, Tsega L. Solomon, and Linda Columbus. Biophysical Journal. 107: 1697-702 (2014). The ability to detect nanosecond backbone dynamics with site-directed spin labeling (SDSL) in soluble proteins has been well established. However, for membrane proteins, the nitroxide appears to have more interactions with the protein surface, potentially hindering the sensitivity [...]